Characterization of qa-2 mutants of Neurospora crassa by genetic, enzymatic, and immunological techniques
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چکیده
منابع مشابه
Characterization of qa-2 mutants of Neurospora crassa by genetic, enzymatic, and immunological techniques.
Genetic and complementation mapping studies using 20 qa-2 mutants defective for catabolic dehydroquinase indicate that the qa-2 gene encodes a single polypeptide chain and is the structural gene for catabolic dehydroquinase, a 220,000-molecular-weight protein composed of identical 10,000-molecular-weight subunits. Many qa-2 mutants are capable of reversion, but no evidence has yet been obtained...
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The qa-3 gene, one of the four genes in the qa gene cluster, encodes quinate (shikimate) dehydrogenase (quinate: NAD oxidoreductase, ER 1.1.1.24), the first enzyme in the inducible quinic acid catabolic pathway in Neurospora crassa. Genetic analyses have localized 26 qa-3 mutants at 11 sites on the aq-3 genetic map on the basis of prototroph frequencies. Certain mutants, e.g., 336-3-10 and 336-...
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The pyrimidine-3 mutants of Neurospora crassa have received considerable attention in the past. Mitchell and Mitchell' described experiments, using pyr-3a (37301), 3b (37815; temperature sensitive), and 3d (45502), which indicated that the pyr-3a and 3b mutations represent a genetic unit distinct from 3d. The evidence for this view was that pyrimidine-independent heterocaryons could be formed b...
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The ability of Neurospora crassa FGSC 4335 in the biotransformation of hydrocortisone was investigated. The microorganism produced two major metabolites after incubation with the substrate for seven days. Each microbial product was purified chromatographically and identified on the basis of spectral data. The products were identified as 11?,17?,20?,21-tetrahydroxypregn-4-en-3-one (II) and 11?-h...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1977
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.129.1.166-172.1977